Dynamic expression of insulin-like growth factor-binding proteins during chondrogenesis
10.3760/cma.j.issn.1009-4598.2018.05.015
- VernacularTitle: Igfbps在软骨细胞分化过程中的动态表达研究
- Author:
Tengfei WANG
1
;
Yi WU
;
Jing PAN
;
Xia LIU
;
Ran XIAO
Author Information
1. Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100144, China
- Publication Type:Journal Article
- Keywords:
Insulin-like growth factor;
Binding proteins;
Cartilage;
Cell proliferation;
Cell differentiation
- From:
Chinese Journal of Plastic Surgery
2018;34(5):391-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clarify the status of proliferation and differentiation of RCJ3.1C5.18 cells in chondrogenesis and explore insulin-like growth factor binding proteins (Igfbps) gene expression profile during the process. The results provided a research foundation for understanding the role of Igfbps in chondrogenesis.
Methods:Fetal rat derived mesenchymal chondrogenic cell line RCJ3.1C5.18 was used, which progresses spontaneously to differentiated growth plate chondrocytes. The proliferation and differentiation status of cells were determined by cell staining, real-time PCR and Muse™ Cell Analyzer at days 1, 4, 7 and 10 of culture respectively. Igfbps gene expression was assessed by real-time PCR.
Results:In the early period (1-4 days), cells proliferated rapidly and the expression of chondrocytes marker genes, such as typeⅡ collagen, SRY-box 9 and aggrecan increased gradually. Igfbp4 and Igfbp6 gene expression paralleled the expression of chondrocytes marker genes. On days 7-10 of culture, cells viability decreased and gradually developed hypertrophic-like and osteogenic-like characteristics, which was confirmed by specific staining and gene expression of alkaline phosphatase, matrix metallopeptidase 13.Igfbp1, Igfbp3 and Igfbp5 mRNA levels were up regulated gradually at this stage.
Conclusions:The status of proliferation and differentiation of cells are different in the process of chondrogenesis. Changes in Igfbp gene expression are associated with chondrocyte differentiation, suggesting that they have different role during chondrocyte proliferation and differentiation.
