Isolation and identification of the first D8 genotype measles virus in Liaoning province
10.3760/cma.j.issn.1003-9279.2018.05.007
- VernacularTitle: 辽宁省首例D8基因型麻疹病毒的分离与鉴定
- Author:
Yan WANG
1
;
Shuang HAO
1
;
Wensi WANG
1
;
Yinan HAN
2
;
Xing FANG
1
;
Wenqing YAO
1
Author Information
1. The Institute for Immunization Programme, Liaoning Provincial Center for Disease Control and Prevention, Shenyang 110005, China
2. The Institute for Immunization Programme, Dalian Center for Disease Control and Prevention, Dalian 116021, China
- Publication Type:Journal Article
- Keywords:
Measles virus;
D8 genotype;
Genetic characterization
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(5):484-487
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the gene characterization on the first imported D8 genotype measles virus in Liaoning province.
Methods:In this study, Vero/Slam cells were used to isolate measles viruses from throat swabs. Fragments of the H gene (1854 nucleotides) and N gene (450 nucleotides) were amplified by reverse transcription-polymerase chain reaction (RT-PCR) and the PCR products were sequenced and analyzed.
Results:The measles virus isolates and World Health Organization (WHO) D8 genotype reference strain (MVi/Manchester.GBR/30.94) belonged to the same branch in the genetic relationship tree. The nucleotide homology of the N and H gene was 98.9%. Phylogenetic trees were constructed with reference strains of the genotype D8 measles virus of China downloaded from GenBank. The result showed that the nucleotide similarities between the measles virus isolated in this study and the D8 genotype measles virus prevalent in Hong Kong from 2012 to 2016 and MVi/LosPatios.COL/11.18/D8 in Columbia was 100%.
Conclusions:It is the first time to do surveillance for the D8 genotype measles virus since measles virus surveillance was carried out. It was of great significance to accumulate the bases of measles virus molecular epidemiology in Liaoning province, and helpful to analyze and trace the transmission of measles virus in the whole country and the world.
