Association between prion infection and Ryanodine receptor 2 in scrapie-263 K infected hamster
10.3760/cma.j.issn.1003-9279.2018.05.005
- VernacularTitle: 羊瘙痒因子263K感染仓鼠脑组织中兰尼碱受体2表达变化的研究
- Author:
Yao LI
1
;
Jianle LI
1
;
Kang XIAO
2
;
Yue MA
2
;
Liping GAO
2
;
Jing WANG
2
;
Zhen ZHEN
1
;
Yanjun GUO
1
;
Qi SHI
2
Author Information
1. Department of Neurology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
2. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
- Publication Type:Journal Article
- Keywords:
Prion disease;
Ryanodine Receptors;
Neuron
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(5):474-478
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To classify the expression of RyR2 in the brain of prion infected hamsters.
Methods:Immunohistochemical assays was used to verify that the location of RyR2 in the brain slices of hamster. Assays of the brain samples of intracranial inoculation of scrapie infected hamster (agent 263 K) and normal hamster were tested to evaluate the RyR2 expression by Western Blotting. Immunofluorescent assays were used to verify the co-location between RyR2 and PrP protein.
Results:RyR2 mainly located in cortex and Purkinje cells with parts of which are distributed in thalamus, hippocampus and olfactory bulb. The expression of RyR2 significantly decreased in the 263 K infected hamster at terminal stage. The Immunofluorescence tests showed that RyR2 was colocalized with PrP protein.
Conclusions:The experimental data showed that RyR2 may play a crucial role in prion disease, which might be closely linked to the cognition impairment and neuron loss. The relation between RyR2 and prion disease still needs further research.
