Study on the determination of pyrrole adduct content in hair samples
10.3760/cma.j.issn.1001-9391.2018.05.020
- VernacularTitle: 毛发样本中吡咯加合物含量测定方法的研究
- Author:
Xianjie LI
1
;
Ming LI
;
Yu WANG
;
Rui YANG
;
Qiong WANG
;
Xiaohong ZHANG
;
Keqin XIE
Author Information
1. Institute of Toxicology, School of Public Health, Shandong University, Jinan 250012, China
- Publication Type:Journal Article
- Keywords:
N-hexane;
2, 5-hexandione;
Hair;
Pyrrole adducts
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2018;36(5):382-385
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To find out a method to determine the pyrrole adducts in the hairs.
Methods:Collected the hair from common people and rats, defatted after completely washed, steeped the hair in different concentration of 2, 5-hexandione to build hair model containing pyrrole adducts; dissolved the hair and determined the concentration of pyrrole adducts.
Results:(1) . The combination of 0.72 mol/L of sodium hydrate and 2% tyrisin could dissolve the hair, and the digestion liquid could react with the Ehrlich's reagent showing fuchsia color; (2) . The color could maintain longer after adding more ethanol; (3) . More pyrrole adducts would be produced by the increasing the concentration of 2, 5-dihexandione (P<0.01) ; (4) . Concentration of pyrrole adducts in n-hexane treated hair showed no difference compared with control (P>0.05) .
Conclusion:the method could be used to determine the concentration of pyrrole adducts in hair exposed to n-hexane
