Simultaneous determination of 21 organophosphrous pesticides in human serum by QuEChERS-gas chromatography-mass spectrometry
10.3760/cma.j.issn.1001-9391.2018.05.019
- VernacularTitle: 人血清中有机磷农药QuEChERS法提取的气相色谱-质谱联用法测定
- Author:
Xiaojuan LI
1
;
Yinxia HE
;
Baoli ZHU
Author Information
1. Jiangsu. Provincial Center for Disease Prevention and Control, Nanjing 210009, China
- Publication Type:Journal Article
- Keywords:
QuEChERS;
GC-MS;
Humanserum;
Organophosphrous pesticides
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2018;36(5):378-381
- CountryChina
- Language:Chinese
-
Abstract:
Objective:A method for the simultaneous determination of organophosphrous pesticides inhuman serum was developed.
Methods:Using C18 and PSA as absorbent, 21 organophosphrous pesticides including methamidophos, dichlorvos, acephate, Omethoate, demeton and etc. in human serum were determined by combing QuEChERS with GC-MS, organophosphrous pesticides were qualitatedsimultaneously by retention time and characteristic ions. Their contents were determined by internal standard method of quantitative ions.
Results:The linear range was from0to 0.60μg/ml with a good correlation coefficients (r ≥ 0.9961) . The LODs of this methodranged from 0.0009 to 0.0111 μg/ml. The average recoveries of3 organophosphrous pesticides (methamidophos、dichlorvos and acephate) ranged from 71.8%~85.3%, The average recoveries of other 18 organophosphrous pesticidesranged from 82.9%~102.9%. The relative standard deviations were0.8%~7.0%.
Conclusion:The convenient operation and versatility of this method are suitable forthe fast screening and accurate detection of 21 organophosphrous pesticides in human serum.
