Effects of crystalline silica with different exposure patterns on lung fibrosis in rats
10.3760/cma.j.issn.1001-9391.2018.05.003
- VernacularTitle: 不同模式石英染尘对大鼠肺纤维化的影响
- Author:
Zhe PENG
1
;
Hai ZHANG
;
Yun ZHOU
;
Xiuqing CUI
;
Gangtao SUN
;
Yuewei LIU
Author Information
1. Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Center for Disease Control and Prevention, Wuhan 430069, China
- Publication Type:Journal Article
- Keywords:
Quatz;
Dust;
Pulmonary fibrosis;
Rats
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2018;36(5):331-335
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of crystalline silica with different exposure patterns on lung fibrosis in rats.
Methods:A total of 20 adult male Wistar rats were randomly divided into 4 groups consisting of five animals each and received intratracheal instillation of sterile saline or silica suspension in different patterns: saline once at day 0, saline once/week, crystalline silica 50 mg at day 0, crystalline silica 6.25 mg/week. The rats were sacrificed at 8 weeks. The lung tissues were collected for pathological analyses, and determining mRNA and protein levels of related fibrogenic molecules.
Results:The collagen deposition induced by crystalline silica in lung tissues were increased. The mRNA levels of IL-1β and Col I in group c were significantly elevated than those in group saline once at day 0 (all P<0.05). Compared with group saline once/week, the mRNA levels of IL-1β, TGF-β, Col I, Col III and CTGF were significantly increased in group crystalline silica 6.25 mg/week (P<0.05) . The mRNA levels of Col I and CTGF were significantly increased in group crystalline silica 6.25 mg/week in comparison with those in group crystalline silica 50 mg at day 0 (P<0.05) .
Conclusion:Given the same cumulative dose of crystalline silica, multiple exposures were likely to induce more severe lung fibrosis.
