The effect of pulmonary injury in rats induced by paraquat
10.3760/cma.j.issn.1001-9391.2018.05.002
- VernacularTitle: 七叶皂苷钠对百草枯致大鼠肺损伤的影响
- Author:
Yiwei SU
1
;
Feng LI
;
Yuan ZHAO
;
Yanhua LI
;
Yuchao FENG
;
Yimin LIU
Author Information
1. Guangzhou Prevention and Treatment Center for Occupational disease, Guangzhou 510620, China
- Publication Type:Journal Article
- Keywords:
Paraquat;
Sodium aescinate;
Respiratory distress syndrome;
Pulmonary hydroxyproline
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2018;36(5):327-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of sodium aescinate on the development process of lung injury induced by paraquat.
Methods:Forty-five health adult male SD rats were randomly divided into control group, PQ group, sodium aescinate group, and 15 rats in each group. The PQ group and sodium aescinate group were given a one-time intraperitoneal injection of 18mg/kg body weight of rats PQ, the control group was given the same amout normal saline. Rats in sodium aescinate group were injected 2 mg/kg body weight sodium aescinate into abdominal cavity for 7 days continually, but the same volume of saline was injected into the other groups. Finally, at 7, 14 and 28 days after PQ poisoning, five rats were kills for measuring lung tissue pathological changes and the value of TGF-β1, TNF-α, hydroxyproline in each group.
Results:The expression of TNF-α in serum of 7th day [ (17.03±0.82) ng/ml] and 14th day [ (15.74±0.91) ng/ml] of sodium aescinate group were lower than the corresponding period of PQ groups’, and the difference had statistical significance (P<0.05) . The expression of TGF-β1 in serum of 7th day[ (225.93±8.33) ng/ml], 14th day [ (216.62±9.48) ng/ml] and 28th[ (181.41±6.10) ng/ml] of sodium aescinate group were lower than the corresponding period of PQ groups’, and the difference had statistical significance (P<0.05) . Lung tissue pathological changes showed, compared with control group, inflammatory injury at 7th day and fibrosis degree at 28th of rats’ lung reduced on sodium aescinate group. The expression of hydroxyproline in rats’ lung of 7th day[ (1.246±0.018) μg/mg], 14th day [ (1.269±0.034) μg/mg] and 28th[ (1.283±0.028) μg/mg] of sodium aescinate group were lower than the corresponding period of PQ groups’, and the difference had statistical significance (P<0.05) .
Conclusion:sodium aescinate could reduce the pulmonary inflammatory injury and hydroxyproline value of PQ poisoning rats, so sodium aescinate could ameliorate lung injury induced by PQ.
