Antimicrobial susceptibility and drug-resistance genes of Yersinia spp. of retailed poultry in 4 provinces of China
10.3760/cma.j.issn.0253-9624.2018.04.006
- VernacularTitle: 中国四省份禽肉中耶尔森菌的耐药性及其耐药基因研究
- Author:
Zixin PENG
1
;
Mingyuan ZOU
;
Jin XU
;
Wenying GUAN
;
Ying LI
;
Danru LIU
;
Shuhong ZHANG
;
Qiong HAO
;
Shaofei YAN
;
Wei WANG
;
Dongmin YU
;
Fengqin LI
Author Information
1. Key Laboratory of Food Safety Risk Assessment of Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing 100021, China
- Publication Type:Journal Article
- Keywords:
Yersinia enterocolitica;
Drug resistance;
Drug resistance gene
- From:
Chinese Journal of Preventive Medicine
2018;52(4):358-363
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To monitor the antimicrobial resistance and drug-resistance genes of Yersinia enterocolitis, Y. intermedia and Y. frederiksenii recovered from retailed fresh poultry of 4 provinces of China.
Methods:The susceptibility of 25 isolated Yersinia spp. to 14 classes and 25 kinds of antibiotics was determined by broth microdilution method according to CLSI (Clinical and Laboratory Standards Institute). The antibiotic resistance genes were predicted with antibiotic resistance genes database (ARDB) using whole genome sequences of Yersinia spp.
Results:In all 22 Y. enterocolitis tested, 63.7% (14 isolates), 22.8% (5 isolates), 4.6% and 4.6% of 1 isolates exhibited the resistance to cefoxitin, ampicillin-sulbactam, nitrofurantoin and trimethoprim-sulfamethoxazole, respectively. All the 25 isolates were multi-drug resistant to more than 3 antibiotics, while 64.0% of isolates were resistant to more than 4 antibiotics. A few Y. enterocolitis isolates of this study were intermediate to ceftriaxone and ciprofloxacin. Most Yersinia spp. isolates contained antibiotic resistance genes mdtG, ksgA, bacA, blaA, rosAB and acrB, and 5 isolates recovered from fresh chicken also contained dfrA1, catB2 and ant3ia.
Conclusion:The multi-drug resistant Yersinia spp. isolated from retailed fresh poultry is very serious in the 4 provinces of China, and their contained many kinds of drug-resistance genes.
