Effect of IL-11 on the invasive and metastatic abilities of anaplastic thyroid carcinoma cells
10.3760/cma.j.issn.1673-0860.2018.04.009
- VernacularTitle: 白细胞介素11对甲状腺未分化癌侵袭迁移能力的影响
- Author:
Yu′e JIANG
1
;
Zhaoming ZHONG
2
,
3
;
Xue CHEN
2
;
Wei WANG
2
;
Juanzhang LUO
2
;
Shiqi WANG
2
;
Hongying CHU
2
;
Chuanzheng SUN
2
Author Information
1. Clinical Laboratory, the Third Affiliated Hospital of Kunming Medical University, Kunming 650118, China
2. Department of Head and Neck Surgery, the Third Affiliated Hospital of Kunming Medical University, Kunming 650118, China
3. Department of Oncology, the First Affiliated Hospital of Kunming Medical University, Kunming 650118, China
- Publication Type:Journal Article
- Keywords:
Thyroid neoplasms;
Interleukin-11;
Epithelial mesenchymal transition;
Invasion and metastasis
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2018;53(4):286-291
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of hematopoietic cytokines IL-11 on invasion and metastasis abilities of anaplastic thyroid cacinoma(ATC) cells.
Methods:Real-time PCR was performed for examining the IL-11 mRNA expression in thyroid carcinoma cell lines, and IL-11 protein expression in the supernament of thyroid carcinoma cell lines was detected by ELISA. Molecular cloning was employed to construct IL-11 stable knockdown cell line; MTT assay was used to analyze the effect of IL-11 on the proliferation of ATC cells; Transwell and wound healing assays were employed to analyze the abilities of migration and invasion in ATC cells. Western blotting was used to detect the relative pathway proteins. SPSS statistical package 19.0 was used to analyze the date, and Student′s t test was used for multiple comparisons.
Results:The protein level of IL-11 were significantly lower in knock-down cell lines than that in negative control cell lines(21.55±1.69, 16.18±0.85, 26.37±2.00 vs 54.54±3.99, all P<0.05). Colony formation assays reveal that colony number between knock-down cells and negative control cells has no significance(P>0.05). Meanwhile, MTT assays show that there is no significance between knock-down cell lines and negative control cell line(P>0.05). However, Transwell invasion and migration assays show that number of migrated cells is increased when ATC cells were treated with rhIL-11(0-100 ng/ml)at increasing concentrations.
Conclusion:IL-11 improves the migratory and invasive abilities of ATC cells via inducing EMT of ATC cells, and it can be used as a potential target for ATC molecular targeted therapy.
