Expression and significance of c-fos in resistant cell line TU177/VCR of larynx squamous cell carcinoma
10.3760/cma.j.issn.1673-0860.2018.04.008
- VernacularTitle: c-fos在喉鳞状细胞癌耐药细胞株TU177/VCR中的表达及意义
- Author:
Guodong LI
1
;
Xiaoling HU
2
;
Jianfeng XING
3
;
Ruyi SHI
4
;
Xin LI
1
;
Jianfeng LI
1
;
Tongli LI
1
Author Information
1. Department of Otorhinolaryngology, Shanxi Provincial People′s Hospital Affiliated to Shanxi Medical University, Taiyuan 030012, China
2. Department of Pharmacology, Shanxi Medical University, Taiyuan 030001, China
3. Function Laboratory, Shanxi Medical University, Taiyuan 030001, China
4. Department of Cell Biology and Genetics, Shanxi Medical University, Taiyuan 030001, China
- Publication Type:Journal Article
- Keywords:
Laryngeal neoplasms;
Carcinoma, squamous cell;
Drug resistance, neoplasm;
c-fos;
TU177
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2018;53(4):281-285
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of c-fos on multidrug resistance of laryngeal cancer TU177 cells.
Method:Increasing drug concentration gradient is adopted to establish the stability of the laryngeal cancer drug resistance in cell line; RT-PCR and Western blot were used to detect difference of the c-fos between TU177 and TU177/VCR cells; plasmids with human c-fos knockdown or over expression were transfected into TU177/VCR and TU177 cells respectively, and the effects of different treatment on cell proliferation were investigated with MTT.
Results:The drug resistance of TU177/VCR cells was 26.25-fold in vincristine (VCR), 7.33-fold in Paclitaxel (TAX), 2.41 in cisplatin (DDP), and 5.50 in 5-fluorouracil (5-FU), comparing with TU177( P<0.05). The TU177/VCR cells had significantly higher c-fos expression compared to TU177 cells( P<0.05). The results showed that the IC50 values of 5-FU for the NC group and c-fos shRNA group were (306.2±6.3)μmol/L and (81.3±3.9)μmol/L, respectively, which was decreased by 73% in the c-fos shRNA group compared to that in the NC group (P<0.05). Similarly, the results showed that the IC50 values for 5-FU were (55.3±9.4) μmol/L in NC group and (288.1±7.3)μmol/L in c-fos WT group, which was increased 5.21-fold in c-fos WT cells.
Conclusion:C-fos plays important role in multidrug resistance of larynx cancer cell TU177/VCR, and might become a new molecular target for laryngeal cancer treatment.
