Advances in application of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 system in stem cells research

VernacularTitle: 成簇规律间隔短回文重复序列及其相关蛋白基因9系统在干细胞研究中的应用进展
Author: Sujing SUN ¹ ; Jiahui HUO ; Zhijun GENG ; Xiaoyan SUN ; Xiaobing FU
Author Information

1. College of Life Sciences, General Hospital of PLA, Beijing 100853, China
Publication Type:Review
Keywords: Stem cells; Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated 9; Gene editing
From: Chinese Journal of Burns 2018;34(4):253-256
CountryChina
Language:Chinese
Abstract: Gene engineering has attracted worldwide attention because of its ability of precise location of disease mutations in genome. As a new gene editing technology, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system is simple, fast, and accurate to operate at a specific gene site. It overcomes the long-standing problem of conventional operation. At the same time, stem cells are a good foundation for establishing disease model in vitro. Therefore, it has great significance to combine stem cells with the rapidly developing gene manipulation techniques. In this review, we mainly focus on the mechanism of CRISPR/Cas9 technology and its application in stem cell genomic editing, so as to pave the way for promoting rapid application and development of CRISPR/Cas9 technology.