Eosinophils Modulate CD4+ T Cell Responses via High Mobility Group Box-1 in the Pathogenesis of Asthma.
10.4168/aair.2015.7.2.190
- Author:
Eun Jin SHIM
1
;
Eunyoung CHUN
;
Hyun Seung LEE
;
Bo Ram BANG
;
Sang Heon CHO
;
Kyung Up MIN
;
Heung Woo PARK
Author Information
1. Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea. guinea71@snu.ac.kr
- Publication Type:In Vitro ; Brief Communication
- Keywords:
Asthma;
eosinophil;
high-mobility group box 1 protein (HMGB1);
T cell
- MeSH:
Antibodies;
Asthma*;
Dendritic Cells;
Eosinophils*;
HMGB1 Protein;
Inflammation;
Interleukin-4;
Interleukin-5;
Interleukins;
T-Lymphocytes
- From:Allergy, Asthma & Immunology Research
2015;7(2):190-194
- CountryRepublic of Korea
- Language:English
-
Abstract:
Eosinophils have been reported to modulate T cell responses. Previously, we reported that high-mobility group box 1 protein (HMGB1) played a key role in the pathogenesis of asthma. This study was conducted to test our hypothesis that eosinophils could modulate T cell responses via HMGB1 in the pathogenesis of asthma characterized by eosinophilic airway inflammation. We performed in vitro experiments using eosinophils, dendritic cells (DCs), and CD4+ T cells obtained from a murine model of asthma. The supernatant of the eosinophil culture was found to significantly increase the levels of interleukin (IL)-4 and IL-5 in the supernatant of CD4+ T cells co-cultured with DCs. HMGB1 levels increased in the supernatant of the eosinophil culture stimulated with IL-5. Anti-HMGB1 antibodies significantly attenuated increases of IL-4 and IL-5 levels in the supernatant of CD4+ T cells co-cultured with DCs that were induced by the supernatant of the eosinophil culture. In addition, anti-HMGB1 antibodies significantly attenuated the expressions of activation markers (CD44 and CD69) on CD4+ T cells. Our data suggest that eosinophils modulate CD4+ T cell responses via HMGB1 in the pathogenesis of asthma.
