The expression change of histone H4 acetylation in TCDD-induced cleft palates formation in fetal mice
10.3760/cma.j.issn.1009-4598.2018.04.015
- VernacularTitle: TCDD诱导C57BL/6J胎鼠腭裂过程中组蛋白H4乙酰化的表达
- Author:
Dingwen ZHANG
1
;
Xingang YUAN
;
Yuexian FU
;
Chen WANG
;
Lin QIU
;
Guanghui WEI
Author Information
1. Department of Burn and Plastic Surgery of Children′s Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing Key Laboratory of Child Urogenital Development and Tissue Engineering, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing 400014, China
- Publication Type:Journal Article
- Keywords:
Histone H4;
Acetylation;
2, 3, 7, 8-tetrachlorod -ibenzo-p-dioxin;
Cleft palate
- From:
Chinese Journal of Plastic Surgery
2018;34(4):305-310
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the expression of histone H4 acetylation(Ac-H4) during the cleft palates formation induced by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin(TCDD) in C57BL/6J mice.
Methods:Forty-eight pregnant C57BL/6J mice were completely randomly divided into two groups: ① TCDD group, mice were treated with 20ug/kg of TCDD on gestation day (GD) 10.5 by gastric perfusion; ② control group, mice were treated with an equivalent of corn oil. The head samples were collected and sliced in coronal plane on GD13.5, GD14.5 and GD15.5 respectively. Histone H4 acetylation in the palates were evaluated by immunohistochemical staining and Western Blot in the two groups.
Results:Histone H4 acetylation was mainly expressed in the palatal epithelial cells and slightly expressed in mesenchymal cells. The expression level of histone H4 acetylation was 0.6002±0.2530, 0.9180±0.0941 and 0.8966±0.0908 respectively in control group on GD13.5, GD14.5 and GD15.5; while 1.0229±0.2779, 1.6095±0.2651 and 1.2758±0.1251 in TCDD group. There were statistically significant differences between the control group and TCDD group (P<0.05).
Conclusions:The histone H4 acetylation was involved in the cleft palate formation induced by TCDD in C57BL/6J mice.
