Association between the single nucleotide polymorphism at locus 1165 of β1-adrenoceptor and infection by Enterovirus A71
10.3760/cma.j.issn.1003-9279.2018.04.013
- VernacularTitle: β1-肾上腺素能受体基因编码区1165位单核苷酸多态性与肠道病毒A71感染的关系
- Author:
Bangtao LI
1
;
Zhixian LEI
1
;
Hongai LI
2
;
Lan CUI
3
;
Yuanping HAI
1
;
Ting HUANG
1
;
Wei XIANG
1
Author Information
1. Pediatric Intensive Care, Maternal and Child Health Care Hospital of Hainan Province (Children′s Hospital of Hainan Province), Haikou 570206, China
2. Pediatric Medicine, Hainan Affiliated Hospital of University of South China, Haikou 570206, China
3. Pediatric Medicine, the Affiliated Hospital of Hainan Medical University, Haikou 570206, China
- Publication Type:Journal Article
- Keywords:
β1-adrenoceptor;
Single nucleotide polymorphism;
Enterovirus A71;
Hand, foot and mouth disease
- From:Chinese Journal of Experimental and Clinical Virology
2018;32(4):395-398
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the single nucleotide polymorphism (SNP) at locus 1 165 of β1-adrenoceptor (β1-AR) and to investigate the association between the SNP and the infection by enterovirus A71(EV-A71).
Methods:Polymerase chain reaction (PCR) amplification technique was used to detect the SNP at locus 1 165 of β1-AR between hand, foot and mouth disease (HFMD) and healthy controls by sanger sequencing method .
Results:There was a G1165C SNP and three kinds of genotypes (GG, GC, CC) in β1-AR gene in the 77 cases of EV-A71 HFMD patients and 66 cases of healthy controls. For HFMD patients, frequencies of GG, GC and CC genotypes of the G1165C locus were 10%, 47% and 43%, respectively, and alleles frequency of G and C were 34% and 66%, respectively. But in healthy children, GG, GC, CC genotype frequencies were 7%, 41% and 52%, respectively, and G and C allele frequencies were 28% and 72% respectively. Chi-square analysis showed that there were no significant differences in distribution of genotypes (χ2=1.154, df=2, P=0.562) and alleles frequency (χ2=1.091, df=2, P=0.296) between the EV-A71-infected group and the healthy control group. Between mild and severe EV-A71-infected group, there were no significant differences in distribution of genotypes (χ2=3.945, df=2, P=0.139) and alleles frequency (χ2=3.763, df=2, P=0.052).
Conclusions:The 1 165 SNP in the coding region of β1-AR was not associated with EV-A71 infection and its severity.
