Preliminary study on metabolic symbiosis of HBV-related liver cancer
10.3760/cma.j.issn.1003-9279.2018.04.010
- VernacularTitle: HBV相关肝癌代谢共生初步探讨
- Author:
Jing YANG
1
;
Qinghua MENG
Author Information
1. Department of Severe Liver Diseases, Beijing YouAn Hospital, Capital Medical University, Beijing 100069, China
- Publication Type:Journal Article
- Keywords:
Aerobic glycolysis;
HBV;
Hepatocellular carcinoma;
Tumor metabolic;
Metabolism symbiosis
- From:Chinese Journal of Experimental and Clinical Virology
2018;32(4):382-386
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the glycolysis and metabolic symbiosis of HBV-related liver cancer, and to lay the foundation for studying the metabolism of liver cancer cells.
Methods:10 cases of HBV-related liver cancer patients were collected, IHC detection of metastatic symbiotic markers MCT1, MCT4 for liver cancer and adjacent tissues; choosing HLE liver cancer cell lines, time gradient group use serum-free DMEM medium, divided into 6 h, 12 h, 24 h and 48 h hypoxia cultivating, by western blotting to detect HIF-1α; oxygen concentration gradient group use serum-free DMEM medium, separate culture in three gas incubator with 0.2 % O2, 8% O2 and 19% O2, by western blotting to detect HIF-1α, GLUT1, PKM2, MCT1 and MCT4.
Results:The expression of MCT1 in HBV-related liver cancer tissues was significantly higher than that in adjacent tissues; HLE cell have the highest expression of HIF-1α in the hypoxia cultivating of 12 h. HLE cell have the highest expression of HIF-1α and GLUT1 in hypoxia cultivating of 0.2% O2 and the highest expression of PKM2 in hypoxia cultivating of 8% O2. The oxygen concentration was at 19% MCT1/4 expressed highest.
Conclusions:Abnormal energy metabolism in HBV-related liver cancer tissues; the HLE cell is not preferentially choose the metabolism of aerobic glycolysis in an oxygen-free environment or in the near-constant oxygen environment. In hypoxia environment, aerobic glycolysis may also be associated with metabolic symbiosis.
