Determination of methylenedianiline in urine by high performance liquid chromatography-tandemmass spectrometry
10.3760/cma.j.issn.1001-9391.2018.04.020
- VernacularTitle: 尿中二氨基二苯基甲烷的液相色谱-串联质谱测定方法研究
- Author:
Tiandi LI
1
;
Fen LIU
;
Xingfu PAN
;
Xue TAO
;
Wei ZHAO
;
Huifang YAN
Author Information
1. Shenzhen Prevention and Treatment center for Occupational Diseases, Shenzhen 518001, China
- Publication Type:Journal Article
- Keywords:
Methylenedianiline (MDA);
LC-MS/MS;
Diphenylmethane diisocyanate (MDI);
urine
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2018;36(4):308-311
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a method for determination of metabolites of diphenylmethane diisocyanate (MDI) in urine, i.e. methylenedianiline (MDA) by high performance liquid chromatography-tandem mass (LC-MS-MS) .
Methods:Urine samples were prepared by hydrolyzation with sulfuric acid and extraction by acetonitrile, and then separated on a Shim-pack XR-ODS column, analyzed with high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) . The external solvent standard calibration were tested.
Results:The linearity ranges were 0.05~20.00 μg/L, The related coefficients were 0.999 5. The limit of detection was 0.02 μg/L. The rats of recovery were 91.0%~103.4%. The relative standard deviations were between 2.7%~7.3%.
Conclusion:The method was sensitive, accurate and suitable for the MDA determination in urine of MDI exposed population.
