Construction and pathogenicity of mutant strain ATP binding cassette transporter SSU05_0948 of Streptococcus suis type 2
10.3760/cma.j.issn.1000-6680.2018.04.008
- VernacularTitle: 猪链球菌2型三磷酸腺苷结合蛋白转运蛋白SSU05_0948突变株的构建及其致病性
- Author:
Yaya PIAN
1
;
Zhenxiang GAO
;
Jingjing NIE
;
Ran ZHANG
;
Jihong HU
Author Information
1. National Center for Clinical Laboratories, National Center of Gerontology, Beijing Hospital, Beijing 100730, China
- Publication Type:Journal Article
- Keywords:
Meningitis;
Streptococcus suis type 2;
Gene knockout;
ABC transporter
- From:
Chinese Journal of Infectious Diseases
2018;36(4):234-239
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct the mutant strain ATP binding cassette transporter SSU05_0948 of Streptococcus suis type 2 and comprehensively study its pathogenicity, and to provide useful insights for understanding the mechanism that Streptococcus suis avoid host innate immunity.
Methods:The mutant strain 05ZYH33Δ0948 was constructed through homologous recombination technology. The differences between the mutant strain and the wild type strain were evaluated through bacterial adhesion, whole blood killing, mice meningitis assay, mice and piglets virulence assay. Chi-square test and t test were used.
Results:Successfully constructed the mutant strain 05ZYH33Δ0948. The adhesion results showed that the adhesion rate (0.663±0.047)% of the wild strain to A549 cell was significantly higher than that of the mutant (0.246±0.074)%, the difference was statistically significant (χ2=5.267, P=0.014); the adhesion rate (16.540±2.320)% of the wild strain to Hep2 cell was significantly higher than that of the mutant (1.970±0.320)%, the difference was statistically significant (χ2=0.014, P<0.01); the adhesion rate (5.497±0.174)% of the wild strain to Hep2 cell was significantly higher than that of the mutant (1.950±0.335)%, the difference was statistically significant (χ2=0.016, P<0.01). The killing rate (32.970±3.589)% of the wild strain in whole blood is no difference with the mutant (29.560±3.737)% (χ2=1.200, P=0.133). Piglets competitive infection showed that, the competitive index at 12 h, 24 h and 36 h were 0.046±0.003, 0.107±0.003, 0.064±0.001, respectively. 12 h and 24 h was significant differences(t=15.490, P=0.041), 24 h and 36 h was significant differences(t=5.660, P=0.047), 12 h and 36 h was no differences(t=1.445, P=0.285).
Conclusions:Streptococcus suis type 2 ABC transporter SSU05_0948 is a new adhesion factor and virulence factor of Streptococcus suistype 2, and also a new meningitis factor, which plays important roles in Streptococcus suis against host innate immunity.
