Expression level and clinical significance of LncRNA HOXA11-AS in esophageal squamous cell carcinoma patients
10.3760/cma.j.issn.0253-3766.2018.03.005
- VernacularTitle: 长链非编码RNA HOXA11－AS在食管鳞癌中的表达及意义
- Author:
Xiaoyan SUN
1
;
Xiaofei WANG
1
;
Yuanbo CUI
1
;
Xinguang CAO
2
;
Ruihua ZHAO
3
;
Haiying WEI
1
;
Wei CAO
1
;
Wei WU
4
Author Information
1. Translational Medical Center, Zhengzhou Central Hospital Affiliated Zhengzhou University, Zhengzhou 450007, China
2. Endoscopy Center, Henan Cancer Hospital, Zhengzhou 450008, China
3. Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
4. Department of Medicine, University of California, San Francisco 94118, USA
- Publication Type:Clinical Trail
- Keywords:
Esophageal squamous cell carcinoma;
Long non-coding RNA;
HOXA11-AS;
Prognosis
- From:
Chinese Journal of Oncology
2018;40(3):186-190
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression of long noncoding RNA (lncRNA) HOXA11-AS in esophageal squamous cell carcinoma tissues and the relationship of HOXA11-AS level with clinical outcomes.
Methods:Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to detect the expression level of HOXA11-AS in cell lines HET-1A, EC9706, EC109, and in tumor tissue and paired adjacent tissue samples from 73 ESCC patients who received surgical resection.The correlations of the expression level of HOXA11-AS with clinicopathological features and prognosis were also analyzed.
Results:The relative expression levels of HOXA11-AS in tumor tissue and paired adjacent tissue were 0.832±0.387 and 2.486±1.087, respectively, with significant difference (P<0.001). The expression of HOXA11-AS was upregulated in 63.0%(46/73)ESCC tissues. The relative expression levels of HOXA11-AS in HET-1A, EC-9706 and EC-109 cells were 1.000, 23.553±3.221 and 17.217±1.968, respectively. The expression level of HOXA11-AS was upregulated in ESCC cell lines (P<0.001). High expression level of HOXA11-AS was correlated with histological grade and lymph node metastasis of ESCC patients (P<0.05). However, it was not associated with the age, gender, depth of infiltration and TNM staging (P>0.05). The median overall survival (OS) and median disease-free survival (DFS) of patients with low HOXA11-AS expression were 43 months and 42 months, respectively, significantly longer than 37 months and 28 months of patients with HOXA11-AS high expression (P<0.05). Cox model multivariate analysis showed that the expression of HOXA11-AS and lymph node metastasis were independent factors of poor prognosis of ESCC patients.
Conclusions:The expression of HOXA11-AS is upregulated in esophageal cancer cell lines and tissues. High expression of HOXA11-AS is associated with poor prognosis of ESCC patients.Therefore, LncRNA HOXA11-AS may serve as a predictive marker of postoperative ESCC patients.
