Identification and application of pig model with bilateral external ear defects accompanied by aural atresia
10.3760/cma.j.issn.1009-4598.2018.03.017
- VernacularTitle: 双侧外耳缺失伴发外耳道闭锁猪动物模型的鉴定及其应用
- Author:
Bo PAN
1
;
Ruimin QIAO
;
Meirong YANG
;
Changchen WANG
;
Ye ZHANG
;
Chuan LI
;
Haiyue JIANG
Author Information
1. Plastic Surgery Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100144, China
- Publication Type:Journal Article
- Keywords:
Microtia;
Animal model;
Tempora;
Tomography, spiral computed;
HOXA1 gene
- From:
Chinese Journal of Plastic Surgery
2018;34(3):232-236
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify a pig model with bilateral external ear defects accompanied by aural atresia and investigate its application in plastic surgery.
Methods:Erhualian×Shaziling F2 pig inbreeding population was introduced, and examination of external ear morphology was conducted in all individuals. Temporal computed tomography scanning and mutational detection of HOXA1 gene were conducted in one affected and one normal individuals.
Results:In Erhualian×Shaziling F2 pig inbreeding population, there were 57 normal and 18 affected individuals among the 75 pigs. Affected subjects presented bilateral external ear defects accompanied by aural atresia; temporal computed tomography scanning showed bilateral aural atresia and dysplasiaof middle ear; and gene detection identified homozygous mutation of HOXA1 gene.
Conclusions:Pig model with HOXA1 gene homozygous mutation resembles human microtia at different levels. Our findings provide the theoretical basis for its application to study further pathological mechanism for human microtia.
