Study on virulence related sites based on human enterovirus A71 genome
10.3760/cma.j.issn.1003-9279.2018.03.023
- VernacularTitle: 基于肠道病毒A71型基因组研究其毒力相关位点
- Author:
Jie LI
1
;
Yiwei DU
;
Da HUO
;
Yang YANG
;
Zhichao LIANG
;
Lei JIA
;
Lijuan CHEN
;
Quanyi WANG
Author Information
1. Beijing Center for Disease Control and Prevention, Research Center for Preventive Medicine of Beijing, Beijing 100013, China
- Publication Type:Journal Article
- Keywords:
Enterovirus A71;
Genome;
Virulence
- From:Chinese Journal of Experimental and Clinical Virology
2018;32(3):318-322
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the virulence related risk factors based on the enterovirus A71 (EV-A71) genome.
Methods:The pairwise distance of each section of gene between mild and fatal cases was analyzed. The ⅴ domain of 5′UTR from mild and fatal cases in this study were constructed. Amino acid sequences of EV-A71 were analyzed to find the potential virulence regions which were statistically different between fatal and mild cases.
Results:The two EV-A71 genome sequences in this study belonged to C4a genotype with the genomic homology of 96.2%-97.5%. The nucleotides in the ⅴ domains of the 5 ′UTR of EV-A71 from mild and fatal cases were the same. Each gene of EV-A71 from 31 mild cases and 30 fatal cases shared high homology. A total of four potential virulence sites (2 A: R68 M、2C: K41R、3 A: T/V47 A and 3C: I158 V) which were significantly different between mild cases and fatal cases were obtained.
Conclusions:The four sites in the unstructured protein coding region might be related with the virulence of EV-A71.
