Development of a luminex-based multiplexed immunoassay for detection of IgG antibodies to the viral hemorrhagic fever epidemic in Africa
10.3760/cma.j.issn.1003-9279.2018.03.022
- VernacularTitle: 基于Luminex平台的非洲流行的病毒性出血热IgG抗体多重检测方法的初步建立
- Author:
Wei WU
1
;
Yang LIU
;
Quanfu ZHANG
;
Chuan LI
;
Mifang LIANG
;
Jiandong LI
;
Dexin LI
Author Information
1. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
- Publication Type:Journal Article
- Keywords:
Viral hemorrhagic fever;
Multiplexed immunoassay;
Africa
- From:Chinese Journal of Experimental and Clinical Virology
2018;32(3):314-317
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a multiplexed immunoassay for detection of IgG antibodies against viral hemorrhagic fever epidemic in Africa.
Methods:The recombinant antigens of hemorrhagic fever viruses (HFVs) epidemic in Africa were expressed and purified, and then coupled with the fluorescent microspheres. The coupling effects were evaluated by monoplexed detection of rabbit immune sera. Blood specimens were collected from people from Africa with fever, and multiplexed detection of IgG antibodies to HFVs was performed. Comparison of multiplexed assay and ELISA was performed by paired χ2 test using SPSS software.
Results:Both the purity and concentration of HFVs recombinant antigen met the standards for coupling and detection, and the antigens were successfully coupled with fluorescent microspheres. Seventy-eight sera samples of people from Africa with fever were multiplex detected. Among these, one was tested positive for LASV-specific IgG, one was tested positive for LUJV-specific IgG, 4 were tested positive for DENV-specific IgG and 6 tested positive for YFV-specific IgG. There was no statistically significant difference compared with ELISA, and the two method were highly correlated.
Conclusions:A multiplexed luminex-based immunoassay for detection of IgG antibodies to viral hemorrhagic fever epidemic in Africa was established, which laid the foundation for the differential diagnosis.
