Specificity and application of a norovirus detection method based on receptor capturing
10.3760/cma.j.issn.1003-9279.2018.03.021
- VernacularTitle: 基于受体捕获诺如病毒检测方法的特异性及应用
- Author:
Huiying LI
1
;
Fei WANG
2
;
Dapeng WANG
3
;
Miao JIN
;
Luyang CHENG
2
;
Zhaojun DUAN
1
Author Information
1. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
2. College of Basic Medicine, Chengde Medical University, Chengde 067000, China
3. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
- Publication Type:Journal Article
- Keywords:
Food borne virus;
Norovirus;
ISC-RT-qPCR;
Strawberry
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(3):309-313
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate of the specificity of a new norovirus (NoV) detection method of in situ capture real-time quantitative reverse transcription polymerase chain reaction (ISC-RT-qPCR) and to apply the method for the detection of NoVs in fresh strawberry.
Methods:A panel of stool samples with different NoV genotypes and various inoculums were used for the experiments.
Results:We found that all the tested samples of eight genogroup Ⅱ (GⅡ) NoVs could be detected specifically by ISC-RT-qPCR. Moreover, in contrast to the conventional RT-qPCR method , the situation that the Ct value increased as the inoculum of NoV GⅡ decreased was not shown using ISC-RT-qPCR. When we tested NoVs in strawberry samples by ISC-RT-qPCR, the minimum test limit could reach 1.36 genocopy/10 g of fresh strawberry.
Conclusions:ISC-RT-qPCR is an effective and specific technic and it could be applied for the detection of infectious NoVs from stool samples and fresh strawberry samples.
