Molecular epidemiologic study on norovirus gastroenteritis outbreaks in Zhuhai from 2011 to 2016
10.3760/cma.j.issn.1003-9279.2018.03.014
- VernacularTitle: 珠海市2011—2016年诺如病毒胃肠炎暴发疫情的分子流行病学研究
- Author:
Lirong ZHANG
1
;
Yixiong LIN
;
Hongxia LI
;
Lanlan ZHOU
;
Huitao HUANG
;
Quande WEI
Author Information
1. Zhuhai Municipal Center for Disease Control and Prevention, Zhuhai 519060, China
- Publication Type:Journal Article
- Keywords:
Norovirus;
Outbreak;
Genotype;
Molecular epidemiology
- From:Chinese Journal of Experimental and Clinical Virology
2018;32(3):282-285
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To describe the molecular epidemiological characteristics of norovirus gastroenteritis outbreaks in Zhuhai from 2011 to 2016.
Methods:Anal swab specimens were collected from 576 cases with 56 outbreaks of acute norovirus gastroenteritis from 2011 to 2016. Specimens were tested by real-time RT-PCR. Three to four of norovirus positive specimens were selected from every outbreak to amplify the VP1 gene by RT-PCR and one strain was chosen randomly from every outbreaks to determine the genotype by phylogenetic tree analysis.
Results:Eight genotypes were identified from 56 outbreaks and all of them belonged to GⅡ genogroup. The genotype of norovirus strain changed with prevalence time. The GⅡ.4/2006b was dominant from 2011 to 2012, and replaced by GⅡ.4/Sydney _2012 during the 2012—2013 norovirus season, and both of them never appeared after Feb. 2013. GⅡ.17 was the only genotype during the 2014—2015 norovirus season. All the 7 outbreaks occurred from 2015 to 2016 were caused by GⅡ.3 norovirus. The GⅡ.17and GⅡ.3 were identified from Apr. to Sep. 2016; GⅡ.p16-GⅡ.2 were the only genotype in 12 outbreaks from Nov. to Dec. 2016. The GⅠ genogrope was never identified from 2011 to 2016 in Zhuhai.
Conclusions:The Norovirus GⅡ was the only pathogeny which caused the outbreaks of norovirus gastroenteritis. The recombinant norovirus strain GⅡ.p16-GⅡ.2 emerged and caused large outbreaks in the last two months of 2016 in Zhuhai; several recombinant strains of the GⅡ.p16 RdRp gene were found now, which suggests that attention should be focused on the prevalence and evolution of the recombinant norovirus.
