Loboratory diagnosis and preliminary viral sequence analysis of the first case of human infection with avian influenza A (H5N6) virus in Fujian province

Honghuo Luo; Qi Lin; Jinfeng Zhuang; Linlang DU; Yanhua Zhang; Hongbin Chen; Lin Zhao; Yuwei Weng; Kuicheng Zheng; Jianfeng Xie

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Loboratory diagnosis and preliminary viral sequence analysis of the first case of human infection with avian influenza A (H5N6) virus in Fujian province

VernacularTitle: 福建省首例人感染H5N6禽流感病例实验室诊断与病毒序列初步分析
Author: Honghuo LUO ¹ ; Qi LIN ² ; Jinfeng ZHUANG ¹ ; Linlang DU ¹ ; Yanhua ZHANG ² ; Hongbin CHEN ² ; Lin ZHAO ² ; Yuwei WENG ^{2
,

3} ; Kuicheng ZHENG ^{2
,

3} ; Jianfeng XIE ^{2
,

3}
Author Information

1. Sanming Center for Disease Control and Prevention, Sanming 365000, China
2. Fujian Provincial Center for Disease Control and Prevention, Fujian Key Laboratory for Zoonoses Research, Fuzhou 350001, China
3. School of Public Health, Fujian Medical University, Fuzhou 350001, China
Publication Type:Journal Article
Keywords: Human infected avian influenza H5N6 virus; Laboratory detection; Hemagglutinin and neuraminidase; Phylogenetic tree
From: Chinese Journal of Experimental and Clinical Virology 2018;32(3):263-267
CountryChina
Language:Chinese
Abstract: Objective:To provide effective reference of laboratory detection and prevention-control in avian influenza epidemic via analyzing the detection result of the first case infected avian influenza H5N6 virus in Fujian province.
Methods:The viral RNA was extracted from the patient’s throat swab and specimens of surrounding environment, and detected by real-time RT-PCR. The gene sequences of HA and NA gene segments were obtained by RT-PCR and sequencing, the evolution characteristics of the virus were elementarily analyzed by bioinformatics.
Results:The avian influenza H5N6 virus was confirmed from the patient’s throat swab, termed influenza A/Fujian-Sanyuan/21099/2017(H5N6)virus. The throat swabs of case from 5 different time points were collected and the H5N6 nucleic acid were detected from the first three times collection. Among 43 specimens of surrounding environment, there were 16 H5 virus samples. The HA and NA gene segments of A/Fujian-Sanyuan/21099/2017 were closely related to A/Cygnus atratus/Hubei/2Z2-O/2016(H5N8) and A/chicken/Hubei/ZYSJF16/2016(H5N6), with a similarity of 99.6% and 99.0% respectively. The cleavage site of HA gene contained multiple basic amino acids.
Conclusions:The suspected case was the first case infected with avian influenza H5N6 virus in Fujian province, and the HA and NA genes of virus were highly similar to those of H5N8 and H5N6 virus respectively.