Molecular epidemiological study on hepatitis C virus among drug users in Zhuhai, China
10.3760/cma.j.issn.1003-9279.2018.03.009
- VernacularTitle: 珠海市吸毒人群丙型肝炎病毒分子流行病学研究
- Author:
Hongxia LI
1
;
Quangde WEI
;
Yi ZHOU
Author Information
1. Zhuhai Center for Disease Control and Prevention, Zhuhai 51900, China
- Publication Type:Journal Article
- Keywords:
Drug users;
HCV;
Genotype;
Phylogenetic tree;
Homology
- From:Chinese Journal of Experimental and Clinical Virology
2018;32(3):259-262
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the molecular epidemiology of hepatitis C virus (HCV) among drug users in Zhuhai, China.
Methods:Anti-HCV and HCV RNA were detected by using ELISA and real-time RT-PCR respectively. HCV genotypes and subtypes were determined by direct sequencing of amplicons in partial NS5B regions and BLAST GenBank, followed by phylogenetic analysis.
Results:Of 435 blood plasma from drug users, 242 was negative for anti-HCV and HCV RNA, 147 were positive for anti-HCV and HCV RNA, 46 positive HCV RNA or anti-HCV. The infection rate of HCV was 44.37%. Three genotypes and five subtypes of HCV were detected in 78 samples of positive HCV RNA. Subtypes 6 a, 3b, 3 a, 1b and 1 a, were detected at frequencies of 60.26%, 15.38%, 14.10%, 6.41% and 3.85%, respectively. Compared with strains from Shenzhen, Foshan, Guangzhou, Shanwei, Hong Kong, Denmark, Canada, USA, United Kingdom, India and Nepal, HCV among drug users in Zhuhai were homophylic.
Conclusions:There were homology among HCV in drug users from Zhuhai and strains from some regions and countries. Subtype 6 a was the most predominant. Subtype 1 a and 3 a were discovered in Zhuhai.
