Hepatitis B virus X protein promotes B7-H6 gene activation
10.3760/cma.j.issn.1003-9279.2018.03.008
- VernacularTitle: 乙型肝炎病毒X蛋白对B7-H6基因的激活作用
- Author:
Yong ZOU
1
;
Xiaoan YANG
2
;
Changlong ZHEN
3
;
Xingfei PAN
4
;
Qihuan XU
2
Author Information
1. Department of Blood Transfusion, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
2. Department of Infectious Disease, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
3. Department of Emergency, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
4. Department of Infectious Disease, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China
- Publication Type:Journal Article
- Keywords:
NKP30;
B7-H6;
Promoter;
Hepatitis B virus
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(3):255-258
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the key factor(s) of hepatitis B virus X protein (HBx) promoting B7-H6 gene activation.
Methods:The DNA fragments of the B7-H6 promoter were amplified from the human genomic DNA using polymerase chain reaction(PCR). Products of PCR were digested by KpnI and HindⅢ, and inserted into luciferase reporter vector (pGL3-Basic). The correctness of the recombinant plasmid pGL3-B7-H6 was confirmed by sequencing. Human hepatoma cell line HepG2 were co-transfected with pGL3-B7-H6 and the eukaryotic expression vectors (pCMV-HBs、pCMV-HBc and pCMV-HBx), and the relative luciferase activity was detected.The different dose of HBx expression plasmids were transfected into the HepG2 cells, and the expression of B7-H6 protein were determined by Western blot.
Results:A period of 2.2 Kb of B7-H6 promoter region were successfully cloned into pGL3-Basic, which was confirmed by DNA sequencing. The relative luciferase activity was significantly higher in the cells transfected with pGL3-B7-H6 than that in the cells transfected with the empty vector pGL3-Basic (5.24±1.25 vs. 1.12±0.31, P=0.005). The relative luciferase activity in HepG2 cells co-transfected with pGL3-B7-H6 and pCMV-HBx was remarkably increased compared with the control group (17.60±3.36 vs. 6.73±1.36, P=0.001). Western blot further demonstrated that HBx protein can significantly enhance B7-H6 protein expression.
Conclusions:Hepatitis B Virus X proteins enhanced B7-H6 promoter activity and promoted B7-H6 protein expression.
