Characteristics of distributions of serum neutralizing antibodies to human rhinovirus genotype A21
10.3760/cma.j.issn.1003-9279.2018.03.007
- VernacularTitle: 鼻病毒A21型人群血清中和抗体分布特点
- Author:
Junlin ZHU
1
;
Donghong YANG
2
;
Yan XIAO
1
;
Ying WANG
1
;
Lan CHEN
1
;
Zhancheng GAO
2
;
Lili REN
1
;
Jianwei WANG
1
Author Information
1. Institute of Pathogen Biology, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China
2. Peking University People’s Hosipital, Beijing 100044, China
- Publication Type:Journal Article
- Keywords:
Human rhinovirus A21;
Neutralizing antibody;
Epidemic
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(3):251-254
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Human rhinovirus A21 (HRVA21) with mutations in antigenic gene has been reported causing severe human infections. This study aimed to investigate intensively the pathogenesis of HRVA21 by identifying the characteristics of neutralizing antibodies (NAbs) distribution.
Methods:Virus stock was isolated from HRVA21-positive respiratory specimens. The tissue culture infective dose 50 (TCID50) was applied. Sera from healthy volunteers in different age groups were used to analyze the NAbs by using the diluted serum and fixed viral TCID50.
Results:We obtained the HRVA21 virus stock and its whole genome sequences shared 100% similarity to the used clinical sample. In the 379 collected serum samples, the positive rates of NAbs were 21.7%, 14.1%, 28.2%, 25.4%, 27.9% and 20.7% in age groups of 0-5 y, 5-14 y, 15-25 y, 26-45 y, 46-60 y and > 60 y, respectively. In age groups of >5-14 y and 15-25 y, the positive rate of NAbs showed significantly different (χ2=4.68454, P=0.03044). Higher geometric mean titers were found in 15-25 y group compared to 5-14 y age groups (P=0.0313).
Conclusions:The low level of HRVA21 NAbs in different age groups indicated a low epidemic of HRV21 infection and high susceptability to infections by the virus.
