B1 subgenotype of human metapneumovirus detected in children with acute respiratory infection
10.3760/cma.j.issn.1003-9279.2018.03.006
- VernacularTitle: 急性呼吸道感染患儿中检出B1基因亚型人偏肺病毒
- Author:
Guoquan FAN
1
;
Xiaoli LIU
2
;
Jingjing WEI
3
Author Information
1. Department of Microbiology and Immunology, Basic Medical College of Shanxi Medical University, Taiyuan 030001, China
2. Shanxi Children′s Hospital, Taiyuan 030001, China
3. Department of Pediatrics, Shanxi Medical University, Taiyuan 030001, China
- Publication Type:Journal Article
- Keywords:
Human metapneumovirus;
Nucleoprotein;
Children;
Acute respiratory infection
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(3):247-250
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To reveal the prevalence of human metapneumovirus (HMPV) among pediatric patients with acute respiratory infection (ARI).
Methods:Nasopharyngeal aspirates were collected from hospitalized young children (1-5 years) diagnosed as ARI in Department of Respiratory Disease, Shanxi Provincial Children′s Hospital, Taiyuan city. The nucleoprotein (N) gene of HMPV was amplified by reverse transcription-polymerase chain reaction (RT-PCR). The positive products of PCR were sequenced, made homology and phylogenetic analysis.
Results:One sample collected in Feb. 2017 and two samples collected in Nov. 2017 were HMPV positive in RT-PCR detection. The homologies of nucleic acid and deduced amino acid sequences of the three positive PCR products with the corresponding HMPV sequences in GenBank were over 83% and 90%, respectively. Phylogenetic analysis revealed that HMPV B1 subgenotype was detected.
Conclusions:HMPV B1 subgenotype was the major epidemic HMPV in young children with ARI in Taiyuan city.
