Trichloroethylene-induced abnormal methylation on promoter region of SET in hepatic L-02 cells
10.3760/cma.j.issn.1001-9391.2018.03.002
- VernacularTitle: 三氯乙烯致肝细胞毒性中SET启动子区甲基化水平的改变
- Author:
Jiawen RUAN
1
;
Zhihong CHEN
;
Weixue LU
;
Hang ZHANG
;
Xiaohu REN
;
Xinfeng HUANG
;
Jianhui YUAN
;
Yungang LIU
;
Jianjun LIU
Author Information
1. School of Public Health, Southern Medical of University. 510515 GuangZhou, China Ruan Jiawen and Chen Zhihong contributed equally to the article
- Publication Type:Journal Article
- Keywords:
Trich loroethylene;
L-02 cells;
Patient SE translocation;
DNA methylation
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2018;36(3):165-168
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the trichloroethylene-induced alteration of methylation on the promoter region of SET and related mechanisms in hepatic L-02 cells.
Methods:L-02 cells were treated with different concentrations of TCE(0 mmol/L, 1 mmol/L, 2 mmol/L, 4 mmol/L, 8 mmol/L) for 24 h. The genomic DNA were then extracted and modified by bisulfite sodium. The DNA methylation was then analyzed using bisulfite sequencing PCR (BSP).
Results:The overall methylation on promoter region of SET was decreased along with the increased concentrations of TCE in hepatic L-02 cells. Moreover, 73 CpG islands were found abnormally altered, among which 9 were predicted in transcriptional factor binding regions.
Conclusion:The decreased levels of CpG islands in the transcriptional factor binding region may contribute to the elevation of SET in TCE-induced hepatotoxicity.
