Effects of ascorbic acid on the expression of p53 and Bcl-2 protein in NIH/3T3 cells exposed to nickel
10.3760/cma.j.issn.1001-9391.2018.03.001
- VernacularTitle: 抗坏血酸对镍染毒NIH/3T3细胞P53及Bcl-2蛋白表达的影响
- Author:
Chao TIAN
1
;
Yue WANG
;
Jiping LI
;
Shengyuan WANG
;
Bing XIA
;
Yue YANG
;
Chang LI
;
Qi WANG
;
Yonghui WU
Author Information
1. School of Public Health, Harbin Medical University, Harbin 150081, China
- Publication Type:Journal Article
- Keywords:
Nickel;
Ascorbic acid;
Protein P53
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2018;36(3):161-164
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the protective effect of Ascorbic acid (AA) on the injury of nickel-exposed mouse embryonic fibroblasts (NIH/3T3) .
Methods:A model of damage induced by 50 μg/mL nickel refining dust was established to determine the relative survival rate of cells, superoxide dismutase (SOD) , lactate dehydrogenase (LDH) and glutathione peroxidase. (GSH-Px) activity, hydrogen peroxide (H2O2) and malondialdehyde (MDA) content, and p53 (wild-type) , Bcl-2 protein expression. To investigate the protective effect of different doses of ascorbic acid (25, 50, 100 mmol/L) on nickel-refined dust-induced NIH/3T3 cell injury.
Results:The study showed that ascorbic acid Ⅲ group can make the NIH/3T3 cell survival rate increased significantly; Apoptosis rate was reduced; The vitality of SOD and GSH-Px increased significantly, and the difference was statistically significant (P<0.05) . At the same time, the level of MDA and H2O2 and the activity of extracellular LDH enzyme were significantly reduced, and the difference was statistically significant (P<0.05) . The results showed that nickel refining dust induced cell damage through up-regulation of p53 protein and down-regulation of Bcl-2 protein expression; ascorbic acid interventions, the expression level of Bcl-2 protein in ascorbic acid II and III groups was higher than that of nickel refining dust group, and the difference was statistically significant (P<0.05); The expression level of p53 protein in each dose group of ascorbic acid was lower than that of nickel refined dust group, and the difference was statistically significant (P<0.05).
Conclusion:With the increase of concentration of ascorbic acid, oxidative damage levels, antioxidant enzyme levels, reduce cell apoptosis, reduce expression of p53, increased expression of Bcl-2. It showed that ascorbic acid had protective effect on NIH/3T3 cell injury induced by nickel refining dust.
