Effects of monobutyl phthalate on migration and invasion of mouse Leydig tumor cells
10.3760/cma.j.issn.0253-9624.2018.02.011
- VernacularTitle: 邻苯二甲酸单丁酯对小鼠睾丸间质瘤细胞迁移和侵袭能力的影响
- Author:
Pan GONG
1
;
Hongchao LU
;
Chang ZHANG
;
Shanshan CHEN
;
Yubang WANG
Author Information
1. Department of Safety Assessment and Research Center for Drug, Pesticide and Veterinary Drug of Jiangsu Province, The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China
- Publication Type:Journal Article
- Keywords:
Mice;
Testis;
Monobutyl phthalate;
Epithelial-mesenchymal transition;
Migration
- From:
Chinese Journal of Preventive Medicine
2018;52(2):175-179
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aimed to investigate the influence of monobutyl phthalate (MBP) on the expressions of epithelial-mesenchymal transition (EMT)-related proteins, migration and invasion of mouse Leydig tumor cells (MLTC-1) cells.
Methods:After exposed to different doses of MBP (0、10-7、10-6, 10-5, 10-4, 10-3 mol/L) for 24 h or 48 h, cell viability was determined by 3-(4 5-dimethyl-2-thiazolyl)-2 5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Expressions of vimentin, E-cadherin, N-cadherin and Snail proteins related to EMT were detected by Western blot. The ability of migration and invasion of MLTC-1 were assessed by wound healing assay and Transwell Boyden chamber assay, respectively.
Results:Relative expressions of vimentin, Snail and N-cadherin proteins were promoted ((1.56±0.07) vs (1.78±0.08), (1.22±0.06) vs (1.44±0.07), (1.33±0.11) vs (2.19±0.06), all P values were<0.001) and E-cadherin (0.66±0.09) vs (0.47±0.06), P<0.001,protein was inhibited after the cells stimulated with MBP (0, 10-7 and 10-6 mol/L). The capability of wound closure of MLTC-1 cells were (6.64±2.07)%, (15.61±2.83)%, (39.91±0.33)%, respectively and the invading/migrating cells were (32.67±3.51), (57.67±2.52), (82.67±6.51), respectively, which were obviously increased under MBP treatments (0, 10-7 and 10-6 mol/L) (all P values were <0.001).
Conclusion:Monobutyl phthalate affected the expressions of EMT-related proteins and enhanced the migration and invasion of MLTC-1 cells.
