Relationships between the enrichment of ETBF, Fn, Hp in intestinal and colorectal cancer
10.3760/cma.j.issn.0253-3766.2018.02.004
- VernacularTitle: 肠毒素脆弱拟杆菌具核梭杆菌和幽门螺旋杆菌在肠道内富集度与结直肠癌的关系
- Author:
Jing ZHANG
1
;
Xiaolan LU
1
;
Gang ZHAO
1
;
Haitao SHI
1
;
Yan GENG
2
;
Wenting ZHONG
1
;
Lei DONG
1
Author Information
1. Department of Digestive Disease, the Second Affiliated Hospital of Xi′an Jiaotong University, Xi′an 710004, China
2. Clinical Laboratory, the Second Affiliated Hospital of Xi′an Jiaotong University, Xi′an 710004, China
- Publication Type:Clinical Trail
- Keywords:
Colorectal neoplasms;
Adenoma;
Colonic polyps;
Bacteroides fragilis;
Fusobacterium nucleatum;
Helicobacter pylori
- From:
Chinese Journal of Oncology
2018;40(2):99-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore relationships between the enrichment of ETBF, Fn, Hp in feces, tissues and colorectal cancer.
Methods:Feces, lesion tissue and adjacent tissue from 24 patients with colorectal cancer and 31 patients with adenomas were collected, and we collected Feces and tissue of 20 healthy control persons. Then the copy numbers of enterotoxigenic B. fragilis (ETBF), Fusobacterium nucleatum (Fn) and Helicobacter pylori (Hp) were determined by quantitative real-time PCR. Immunohistochemical method was used to examine the expression intensity of EGFR and p53, and the relationships between different expression intensity of EGFR, p53 and the numbers of three bacterias.
Results:In the feces, copy numbers of ETBF and Fn were as follous: colorectal cancer group>adenomas group>healthy control group (P<0.05). Copy numbers of Hp were as follous: colorectal cancer group>healthy control group (P<0.01); adenomas group>healthy control group (P<0.01). In the tissue, copy numbers of ETBF, Fn were as follows: colorectal cancer group>adenomas group>healthy control group (P<0.05). Copy numbers of Hp were as follows: colorectal cancer group>healthy control group (P<0.01); adenomas group>healthy control group (P<0.01). Copy numbers of those three bacteria in the lesion tissue and the adjacent tissue had no significant difference. This happened both in colorectal cancer group and adenomas group. The different expression intensity of EGFR, p53 and the number of three bacteria showed no obviously statistical correlation(P>0.05).
Conclusion:Adenomatous polyp and colorectal cancer patients show high enrichment of ETBF, Fn and Hp in both feces and tissues. ETBF, Fn and Hp probably contribute to the development of adenomatous polyp and colorectal cancer. Trial registration Chinese Clinical Trial Registry, ChiCTR-BOC-17012509.
