Experimental research in volume retention of different fat-processing techniques
10.3760/cma.j.issn.1009-4598.2018.02.015
- VernacularTitle: 不同纯化方法在脂肪体积保持方面的实验研究
- Author:
Rongwei WU
1
;
Haibin LU
;
Xiaonan YANG
;
Zhenhua JIA
;
Jingyi ZHAO
;
Xiaolei JIN
;
Zuoliang QI
Author Information
1. Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100144, China
- Publication Type:Journal Article
- Keywords:
Adipose tissue;
Fat-processing technique;
Fat content;
Volume retention
- From:
Chinese Journal of Plastic Surgery
2018;34(2):141-145
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To perform a comparative study of different fat-processing techniques to explore which technique could obtain higher fat content.
Methods:Lipoaspirates was purified with three groups of fat-processing techniques (sedimentation, centrifugation, and cotton pad filtering). Isometric purified fat tissue was centrifuged at 5 000 g for 5 minutes to quantify the oil, aqueous, and fat content from them. HE staining was applied to evaluate the effect of different fat-processing techniques on the integrity of adipocyte.
Results:The fat content, aqueous content, and oil content of three groups was respectively 86.0%, 9.6% and 4.4% for the cotton pad filtering group; 74.4%, 14.8% and 10.8% for the centrifugation group; 62.0%, 28.4% and 9.6% for the sedimentation group. In the HE histologic findings, no significant difference was observed in the integrity of adipocyte of three groups (P>0.05).
Conclusions:In comparison with the sedimentation group and the centrifugation group, more fat content, less oil content and aqueous content was observed in the cotton pad filtering group. The cotton pad filtering technique was helpful in postoperative volume retention.
