Expression of TNF-α and PCNA in human breast tissue injected with polyacrylamide hydrogel and clinical significance
10.3760/cma.j.issn.1009-4598.2018.02.014
- VernacularTitle: TNF-α和PCNA在聚丙烯酰胺水凝胶注射隆乳术后乳腺组织中的表达
- Author:
Zhaodi XIE
1
;
Guoqian YIN
;
Siding LU
;
Zixiang CHEN
;
Jiangying ZHU
Author Information
1. Department of Plastic Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China
- Publication Type:Journal Article
- Keywords:
Polyacrylamide hydrogel;
Tumor necrosis factor-α
;
Proliferating cell nuclear antigen;
Augmentation mammolpalsty;
Immuno histochemistry
- From:
Chinese Journal of Plastic Surgery
2018;34(2):137-141
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of TNF-α and PCNA in human breast tissue with polyacrylamide hydrogel(PAHG) injection, and to provide the initial theory basis for its prognosis and clinical treatment.
Methods:Immunohistochemistry was used to measure the expression of tumor necrosis factor α(TNF-α)and proliferating cell nuclear antigen (PCNA) in 20 normal breast tissues and 40 cases with PAHG injection, analysis was also done by HE staining.
Results:①HE staining showed that there were a large number of homogeneous amorphous gel-like injections under optical microscope. Around PAHG there were different degrees of fibrous tissue hyperplasia with or without fibrous degeneration and lots of inflammatory cells. Local foreign body giant cell reaction and ductal dilatation also can be seen around PAHG. ②The IA levels of TNF-α and PCNA in the experimental group were 3.9± 0.3 and 3.2 ± 0.2, the IA levels of TNF-α and PCNA in the control group were 1.0 ± 0.1 and 1.3 ± 0.3, the IA level in the experimental group was significantly higher than that in the control group (P=0.000). The difference was statistically significant. The positive rates of TNF-α and PCNA in the experimental group were 90% (36/40)and 85% (34/40)respectively; the positive rates of TNF-α and PCNA in the control group were 30% (6/20), 40% (8/20). The positive expression rate in the experimental group was significantly higher than that in the control group (P=0.000). The difference was statistically significant. TNF-α and PCNA has Pearson positive relevance in the changes(r=0.3, P=0.040), the difference was statistically significant.
Conclusions:TNF-α and PCNA are involved in the immunoreaction of mammalian breast tissue injected with polyacrylamide hydrogel, which may induce the aseptic inflammation, fibrous tissue hyperplasia and related issues through mutual influence.
