Complete genomic sequence analysis of a GV Murine Norovirus in wild mice
10.3760/cma.j.issn.1003-9279.2018.01.000
- VernacularTitle: 1株野生鼠诺如病毒的全基因组序列测定及分析
- Author:
Mengmeng LIU
1
;
Lili LI
2
;
Lin LIN
3
,
4
;
Xiaofeng WANG
1
;
Zhaojun DUAN
2
Author Information
1. Department of Medical Microbiology, Qingdao University Medical College, Qingdao 266021, China
2. Department of Diarrhea, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
3. Shandong Provincial Center for Disease Control and Prevention, Academy of Preventive Medicine of Shandong University, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Jinan 250014
4. China
- Publication Type:Journal Article
- Keywords:
Murine Noroviruses (MNV);
Whole genome;
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(2):176-180
- CountryChina
- Language:Chinese
-
Abstract:
0bjective To identify the molecular structure and genome homology characteristics of a GV murine norovirus (MNV) from wild mice.Methods:Nucleic acids were extracted from collected mouse intestinal specimens and subjected to high-throughput sequencing. The whole genome sequence was obtained by RT-PCR and RACE method .
Results:The MNV genome was 7, 832 bp in length, with 76.8%-78.1% nucleotide identity to other selected murine norovirus strains in GenBank. The P2 region, the hypervariable area in viral protein 1 gene shows highest heterology, and the identity was among 60.9%-78.1%, lower than other genes regions. The result demonstrated the higher variability of this newly isolated WNV strain.
Conclusions:The wild-type MNV isolated in this study is first reported in China, which enriched the domestic MNV gene pool and provided a reference for understanding the molecular genetic characteristics and evolutionary origin of MNV in China.
