Genotyping and molecular tracing of the first local infection of dengue virus in Taizhou
- VernacularTitle: 台州市本地感染登革病毒分子分型及溯源分析
- Author:
Donghong WANG
1
;
Danhong QIU
1
;
Jian WENG
1
;
Ying SHENG
1
;
Haijiang LIN
1
;
Chunping LIN
2
;
Chao KONG
2
;
Xiaojing ZHOU
2
;
Weiwei SHEN
1
Author Information
- Publication Type:Journal Article
- Keywords: Dengue virus; E gene; Phylogenetic tree
- From: Chinese Journal of Experimental and Clinical Virology 2018;32(2):169-172
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the first dengue fever case in Taizhou and trace probable transmission sources.
Methods:Collected serum of three patients for antigen, antibody and nucleic acid detection. Dengue viruses were isolated and cultured in C6/36 cell. The whole length of E gene was amplified by reverse transcriptase-polymerase chain reaction(RT-PCR) and then sequenced. The phylogenetic tree was drawn.
Results:Three cases were positive in nucleic acid detection. Two cases were IgM positive. One case was NSI antigen postive. Three strains of type I dengue virus were isolated from samples. The phylogenetic trees shown that the three strains were on the same branch. The identities of nucleotide were 99.87%. The identities of amino acid were 99.6%-99.8%.
Conclusions:The dengue virus strains isolated in Taizhou was imported from Guangdong or Southeast Asia and caused location infection.
