Detection and clinical significance of differentially expressed microRNAs in chronic hepatitis B patients before being treated with pegylated interferon

Yanlin Yang; Ming Liu; Ying Deng; Yan Guo; Xuqing Zhang; Dedong Xiang; Li Jiang; Zhonglan You; Yi WU; Maoshi LI; Qing Mao

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Detection and clinical significance of differentially expressed microRNAs in chronic hepatitis B patients before being treated with pegylated interferon

VernacularTitle: CHB患者PegIFN治疗前差异表达microRNAs的检测及意义
Author: Yanlin YANG ^{1
,

2} ; Ming LIU ; Ying DENG ; Yan GUO ; Xuqing ZHANG ; Dedong XIANG ; Li JIANG ; Zhonglan YOU ; Yi WU ; Maoshi LI ; Qing MAO
Author Information

1. Institute of Infectious Disease, Southwest Hospital, Army Medical University, Third Military Medical University
2. the Chongqing Key Laboratory for Research of Infectious Diseases, Chongqing 400038, China
Publication Type:Journal Article
Keywords: Pegylated interferon; Peripheral blood mononuclear cells; MicroRNAs; HBsAg
From: Chinese Journal of Experimental and Clinical Virology 2018;32(2):155-159
CountryChina
Language:Chinese
Abstract: Objective:To detect differentially expressed microRNAs in chronic hepatitis B (CHB) before being treated with pegylated interferon (PegIFN) and the relationship between their target genes and HBsAg loss.
Methods:Pretreatment differentially expressed microRNAs between different response groups were screened using high throughput microarrays and validated by quantitative reverse-transcription polymerase chain reaction (RT-qPCR). Bioinformatics analysis was performed to determine their target genes potential mechanistic roles.
Results:A total of 417 microRNA were differentially expressed between different response groups, among which 342 were up-regulated and 75 were down-regulated. miR-3960, miR-126-3p, miR-23 a-3p and miR-335-5p were verified to be down-regulated by RT-qPCR result in HBsAg loss group. Bioinformatic analysis result show that the relevant pathways of microRNAs include AMPK signal pathway, NOD-like signal pathway, NF-kappa B signal pathway and mTOR signal pathway.
Conclusions:HBsAg loss is probably achieved as the result of genes expression regulated in association with immune response, further enhance the immune response of HBV elimination and acquire HBsAg loss.