Detection of human Torque teno virus in serum sample of patients with fever of unknown origin by next generation sequencing
10.3760/cma.j.issn.1003-9279.2018.02.008
- VernacularTitle: 利用二代测序技术在不明原因发热的血液样本中检测到人细环病毒
- Author:
Yi ZHANG
1
;
Wei WU
;
Ji WANG
;
Xinxin SHEN
;
Xiaozhou HE
;
Mengjie YANG
;
Xuejun MA
Author Information
1. Department of Core Facility, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
- Publication Type:Journal Article
- Keywords:
Fever of unknown origin;
Next generation of sequencing;
Metagenomic;
Torque teno virus
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(2):145-149
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clarify the potential pathogen for fever of unknown origin (FUO) in serum samples for which pathogenic agents were hardly identified with conventional exainatins.
Methods:Random capturing the nucleic acid of pathogen was performed by utilizing the property of sequence non-dependence of next generation sequencing (NGS), followed by enrichment of nucleic acid with multiple displacement amplification (MDA). After sequencing, metagenomic analysis was applied to the raw data and the phylogenetic tree was built to identify the potential pathogen.
Results:The result did not indicate common pathogens for FUO but showed the existence of Torque teno Viurs (TTV). Assembly was carried out to all sequencing reads. The coverage of consensus sequence on reference was calculated. Phylogenetic result indicated that all confidence sequences belonged to 3 genera (α TTV, β TTV and γ TTV).
Conclusions:The characteristics of genome, phylogenesis of TTV and TTV as signal at immunology level were analyzed and clarified. The possible explanation for detection of TTV in 3 genera may be that TTV itself or non-infectious factors caused the immunosuppression, which finally result e in rise of TTV detection.
