Genomic sequence analysis of a hantavirus found in Yunnan bat
10.3760/cma.j.issn.1003-9279.2018.02.007
- VernacularTitle: 云南蝙蝠携带新型汉坦病毒及其基因组分析
- Author:
Hongyan DENG
1
;
Jinglin WANG
2
;
Lili LI
3
;
Yunyun XIN
4
;
Mengmeng LIU
1
;
Yun WANG
1
;
Zhaojun DUAN
3
Author Information
1. Department of Pathogeny Biology, Qingdao University Medical College, Qingdao, 266021 China
2. Yunnan Animal Science and Veterinary Institute/Yunnan Tropical and Subtropical Animal Virus Disease Key Laboratory, Kunming, 650224
3. National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052
4. Children Medical Center, The First Affiliated Hospital of Hunan Normal University, Changsha, 410003 China
- Publication Type:Journal Article
- Keywords:
Bats;
Hantavirus;
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(2):140-144
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the novel bat-borne hantavirus on Yunnan province.
Methods:Eighty-four bats were collected from Puer, Yunnan from July to August in 2016. A hantavirus, named as DodeHV, was identified in Pomona Roundleaf Bat by high-throughput sequencing technology. Nested or Semi-nested reverse transcription-polymerase chain reaction was used to confirm and screen DodeHV, as well as to amplify its genome sequence by specific primers. Then homology and phylogenetic analysis of all three segments were conducted by using software MegAlign and MEGA 6.0.
Results:All the positive individuals are Pomona Roundleaf bats by PCR in this study and 4 out of 67 (5.97%) Pomona Roundleaf bats were positive. The complete ORF of DodeHV S Segment, the most sequence of M and the complete sequence of L segment were obtained. It shared the highest homology with the strain XSV-VN1982B4 found on Phu Tho Province, Viet- Nam in 2013, the nucleotide sequence identities of S, M, L segment compared with XSV was 79.0%, 79.2%, 79.9% respectively, and its amino acid sequence identities was 93.4%, 94.8%, 96.6% respectively. Meanwhile, phylogenetic analysis showed that DodeHV was also closely related to strain XSV-VN1982B4.
Conclusions:The discovery of DodeHV enriched the virus reservoir of our country which is meaningful to public health. It is indicated that there is potential risk of bat-borne DodeHV at cross-border infection at frontier.
