Characterization and analysis of VH1-2-encoded heterosubtypic antibodies isolated from an avian H5N1 patient.
10.3760/cma.j.issn.1003-9279.2018.02.006
- VernacularTitle: 来源于H5N1患者的VH1-2编码广谱抗体的筛选与功能研究
- Author:
Ying SUN
1
;
Tian BAI
;
Zi LI
;
Feier RUAN
;
Lingling CHEN
;
Jian LU
;
Liqi LIU
;
Dayan WANG
;
Yuelong SHU
;
Kun QIN
;
Jianfang ZHOU
Author Information
1. Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
- Publication Type:Journal Article
- Keywords:
Heterosubtypic antibody;
VH1-2;
Influenza virus;
Hemagglutinin
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(2):136-139
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To isolate the cross-reactive antibodies against hemagglutinin of influenza virus and identify its biological function.
Methods:The antibodies gene reservoir of cross-reactive and H5N1 pseudotype particles neutralizing B cell circulating in peripheral blood of a human H5N1 case was recovered by in vitro B cell culture, screening, RT-PCR and expression vector cloning techniques. The Ab gene pairing was screened by transient transfection of human kidney 293T cells and detected using ELISA and neutralization test. The heterosubtypic antibodies were prepared and characterized.
Results:We discovered the VH1-2-based heterosubtypic antibodies from two B cell lineages could neutralize GX-H5N1 pseudotype particles and have broader binding with Group 1 (including H1, H5, H6 and H9) and H7 subtype.
Conclusions:Cross-reactive antibodies can be induced by H5N1 infection.
