Preliminary exploration of replication-defective mechanism of highly attenuated NTV strain of vaccinia virus Tiantan
10.3760/cma.j.issn.1003-9279.2018.02.003
- VernacularTitle: 复制缺陷型痘苗病毒天坛株复制缺陷机制初步探究
- Author:
Panpan HUANG
1
;
Li ZHAO
;
Jiao REN
;
Ying ZHAO
;
Li RUAN
;
Wenjie TAN
;
Houwen TIAN
Author Information
1. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
- Publication Type:Journal Article
- Keywords:
Replication-defective vaccinia virus;
Vaccinia virus vector;
NTV;
Replication-defective mechanism
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(2):119-123
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the expression level of early and late protein of vaccinia virus and to preliminarily explore replication-defective mechanism of highly attenuated NTV strain of vaccinia virus Tiantan.
Methods:We constructed prokaryotic expression vector, expressed and purified homologous early protein E3 and late protein A27 closely related to replication and prepared mouse polyclonal antiserum by immunizing mice with homologous proteins. Early and late protein expression levels of NTV were detected.
Results:We have expressed and purified vaccinia virus proteins respectively in E. coli expression system and prepared homologous mouse polyclonal antiserum. Early protein E3 and late protein A27 could be highly efficient expression in NTV infected non-permissive Hela cells, while expression of late protein F17 was blocked detected by Western blot.
Conclusions:The expression limitation of late protein F17 may be an explanation for the replication-defective mechanism of NTV.
