Brain derived neurotrophic factor enhances the role of mesenchymal stem cells in inhibiting follicular helper T cells
10.3760/cma.j.issn.0253-2727.2018.01.008
- VernacularTitle: 脑源性神经营养因子增强间充质干细胞抑制滤泡辅助性T细胞的作用
- Author:
Sainan YANG
1
;
Xin PU
;
Shali XIANG
;
Jieping CHEN
;
Li PEI
Author Information
1. Department of Hematology, Southwest Hospital, Army Medical University, Chongqing 400038, China
- Publication Type:Journal Article
- Keywords:
Brain derived neurotrophic factor;
Mesenchymal stem cell;
T-lymphocyte subset
- From:
Chinese Journal of Hematology
2018;39(1):37-40
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of brain derived neurotrophic factor (BDNF) on mesenchymal stem cells (MSC) inhibiting follicular helper T cells (Tfh cells).
Methods:The contents of indoleamine 2,3-dioxygenase (IDO), IL-10, TGF-β and IL-21 in MSC culture supernatant were detected by ELISA; The peripheral blood of healthy volunteers were collected, and lymphocyte in peripheral blood was separated by human lymphocyte separation solution; Co-cultures of MSC and lymphocyte were performed by Transwell chamber, and the proportion of CD4+CXCR5+ Tfh cells and their subtypes were detected by flow cytometry.
Results:①The concentrations of IL-10, TGF-β, and IDO in the supernatant of BDNF group (BDNF-stimulated MSC) were higher than those of the control ones (adding PBS with the same volume) [IL-10: (42.1±4.4) ng/ml vs (19.3±2.1) ng/ml, t=4.761, P=0.009; TGF-β: (13.9±1.7) ng/ml vs (5.3±0.6) ng/ml, t=5.129, P=0.008; IDO: (441.3±56.9) ng/ml vs (226.7±37.6) ng/ml, t=3.130, P=0.035]; ②The comparisons between BDNF (co-culture of lymphocyte and BDNF-stimulated MSC) and MSC groups (co-culture of lymphocyte and MSC) were detailed as of follows: the proportion of CD4+ CXCR5+Tfh cells were lower [(3.37±0.21)% vs (6.51±0.27)%, t=9.353, P<0.001], the proportion of CD4+ CXCR5+CXCR3+ CCR6- Tfh cells were higher [(41.14±2.04)% vs (26.72±2.57)%, t=4.383, P=0.012], CD4+CXCR5+CXCR3-CCR6- Tfh2 cells and CD4+CXCR5+CXCR3-CCR6+ Tfh17 cells were lower [Tfh2: (30.16±5.38)% vs (43.26±4.11)%, t=4.426, P=0.012; Tfh17: (15.61±1.52)% vs (22.32±0.72)%, t=4.202, P=0.014], the proportion of CD4+CXCR5+Foxp3+ Tfr cells were higher [(4.95±0.22)% vs (2.32±0.16)%, t=10.241, P<0.001], the concentration of IL-21 in the lymphocyte supernatant was lower [(0.28±0.03) ng/ml vs (0.85±0.08) ng/ml, t=6.675, P=0.003].
Conclusion:BDNF could enhance the inhibitory effect of MSC on Tfh cells through inhibiting the increasing of Tfh cells and the differentiations of Tfh2 and Tfh17 cells.
