Identification and analysis of the proteins interacted with Prestin in cochlear outer hair cells of guinea pig
10.3760/cma.j.issn.1673-0860.2018.01.007
- VernacularTitle: 豚鼠耳蜗外毛细胞Prestin互作蛋白的识别与分析
- Author:
Xuan LUO
1
;
Junyi WANG
1
;
Feiling ZHANG
1
;
Yun XIA
1
Author Information
1. Department of Labor Health and Environmental Hygiene, School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China
- Publication Type:Journal Article
- Keywords:
Prestin;
Hair cells, auditory, outer;
Protein-protein interaction;
Computational biology;
Guinea pigs
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2018;53(1):34-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the regulation and mechanism of Prestin protein by identifying the proteins interacted with Prestin in cochlear outer hair cell(OHC) and analyzing their biological function.
Methods:Co-immunoprecipitation combined mass spectrometry technology was used to isolate and identify the proteins interacted with Prestin protein of OHC, bioinformatics was used to construct Prestin protein interaction network. The proteins interacted with Prestin in OHC of guinea pig were determined by matching primary interaction mass spectrometry with protein interaction network, and annotated their functions.
Results:The results of co-immunoprecipitation combined with mass spectrometry showed that 116 kinds of credible proteins could interact with Prestin. By constructing Prestin protein interaction network, matching the results of mass spectrometry and analyzing of sub-cellular localization, eight kinds of proteins were confirmed that they interacted with Prestin directly, namely EEF2, HSP90AB1, FN1, FLNA, EEF1A1, HSP90B1, ATP5A1, and ERH, respectively, which were mainly involved in the synthesis and transportation, transmembrane folding and localization, structural stability and signal transduction of Prestin protein.
Conclusion:EEF2, HSP90AB1, FN1, FLNA, EEF1A1, HSP90B1, ATP5A1 and ERH provide molecular basis for sensory amplification function of OHCs by participating in biotransformation, transmembrane folding and localization, signal transduction and other biological processes of Prestin protein.
