Protein expression and receptor binding specificity of porcine P19 rotavirus VP8*
10.3760/cma.j.issn.1003-9279.2018.01.019
- VernacularTitle: 猪源P[19]轮状病毒VP8*蛋白的表达及受体结合特征
- Author:
Lihong WANG
1
;
Xiaoman SUN
2
;
Dandi LI
2
;
Hongling WEN
1
;
Zhaojun DUAN
2
Author Information
1. Department of Virology, School of Public Health, Shandong University, Jinan 250012, China
2. National Institute for Viral Disease Control and Prevention, Chinese Center for Diseases Control and Prevention, Beijing 102206, China
- Publication Type:Journal Article
- Keywords:
Rotavirus;
VP8*;
Histo-blood group antigen;
Mucin;
Receptor binding
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(1):89-92
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the receptor binding specificity of VP8* protein of porcine P[19] rotaviruses (RVs) with oligosaccharides.
Methods:The porcine P[19] VP8* protein was expressed and purified. The receptor binding specificity of P[19] VP8* was analyzed by oligosaccharide binding and saliva binding assay.
Results:The P[19] VP8* protein showed significant binding to mucin cores, especially mucin core 2.
Conclusions:Mucin core 2 may be a potential receptor for the porcine P[19] RV, which provides certain basis for the study of virus infection mechanism and RV surveillance.
