Methylation of forkhead box protein 3 gene promoter in CD4+ T cells in patients with chronic hepatitis B
10.3760/cma.j.issn.1003-9279.2018.01.007
- VernacularTitle: 慢性乙型肝炎患者CD4＋T细胞Foxp3基因甲基化状态的研究
- Author:
Jun ZHANG
1
;
Feng LI
1
;
Yuchen FAN
1
,
2
;
Jing ZHAO
1
;
Haiming LI
1
;
Xinyuan LIU
1
;
Mingming TIAN
1
;
Shuai GAO
1
;
Yanbo YU
3
;
Kai WANG
1
,
2
Author Information
1. Department of Hepatology, Qilu Hospital of Shandong University, Jinan 250012, China
2. Institute of Hepatology, Shandong University, Jinan 250012, China
3. Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan 250012, China
- Publication Type:Journal Article
- Keywords:
Forkhead box protein 3;
methylation;
chronic hepatitis B;
CD4+ 25+ Foxp3+ regulatory T cells
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(1):32-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the methylation status and expression of FOXP3 in CD4+ T cells of patients with chronic hepatitis B (CHB).
Methods:Peripheral blood mononuclear cells (PBMCs) from 59 CHB patients and 22 healthy controls (HC) were collected. The percentage of CD4+ CD25+ Foxp3+ Tregs in CD4+ T cells was estimated by flow cytometry. FOXP3 expression was measured by quantitative real time-polymerase chain reaction (RT-qPCR) and Western blotting. The methylation status of FOXP3 was determined by methylation-specific polymerase chain reaction.
Results:The percentage of CD4+ CD25+ pFoxp3+ Tregs in CD4+ T cells, FOXP3 mRNA and protein expression levels were significantly higher in patients with CHB than HCs (P<0.05). Meanwhile, the methylation frequency of FOXP3 was significantly lower in CHB patients than HCs (P<0.05). FOXP3 mRNA levels and the percentage of CD4+ CD25+ Foxp3+ Tregs were significantly lower (P<0.05) in patients with gene methylation than those without.
Conclusions:Aberrant demethylation of FOXP3 gene existed in CD4+ T cells of CHB, which contributed to an elevation in FOXP3 expression and percentage of CD4+ CD25+ Foxp3+ Tregs. It might provide a new target for prevention and treatment of CHB.
