Correlation between caspase-3/6 levels and EBV-induced liver injury in children
10.3760/cma.j.issn.1003-9279.2018.01.004
- VernacularTitle: 儿童原发性EB病毒感染caspase-3/6水平与肝损害的相关性研究
- Author:
Zhengwang WEN
1
;
Yiping CHEN
1
;
Zhiwei XU
1
;
Haifan SHI
1
;
Junbo DI
1
;
Junya CHEN
1
;
Jie CHEN
1
;
Xiaohong CAI
2
Author Information
1. Department of Pediatric Infectious Diseases, the Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325000, China
2. Department of Pediatric Respiratory Diseases, the Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325000, China
- Publication Type:Journal Article
- Keywords:
Epstein-barr virus;
Hepatitis;
Caspase
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(1):17-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship between the EBV-induced liver injury and caspase-3/6 in children.
Methods:Data of 249 patients seen from July 2016 to June 2017 who got infection with EBV were collected in second affiliated hospital of Wenzhou Medical University and the patients were divided into two groups; 168 patients who were diagnosed with hepatitis were selected for abnormal liver function group, laboratory tests were performed in acute phase and convalescent phase. Meanwhile the 81 patients, whose liver function were normal were selected for normal liver function group. Two ml of blood plasma was collected from each patient from both groups at the beginning and after a week’s treatment of the abnormal liver function group. The patients were aged from 1 to 14 years. The abnormal liver function group was further divided into four groups: young children (1-3 years old), preschool children (4-6 years old), school children (7-10 years old), teenagers (10-14 years old). Firstly, we recorded their alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), gamma glutamyl transpeptidase (GGT) and direct bilirubin (DBIL), then by the use of enzyme linked immunosorbent assay (ELISA) we measured the protein level of caspase-3 and caspase-6.
Results:In the group of abnormal liver function group, in the acute phase of the teenagers the level of ALT, caspase-3 and caspase-6 are improved significantly(927.2±82.5 vs. 158.5±41.4, P<0.001, 169.8±52.8 vs. 91.5±10.1, P<0.001, P<0.05, 82.2± 2.6, P<0.001, 965.8±31.5, P<0.001, 184.8±14.3, P<0.001), while the AST, TBLL, GGT and DBLL had no significant difference.
Conclusions:In the process of EBV-induced liver injury, there was a linear correlation between the increase of caspase-3/6 and ALT in the acute stage, suggesting that EBV-induced liver injury is closely related to caspase-3/6.
