Vascular endothelial growth factor-loaded microspheres promote dental pulp regeneration and vascularization
10.3760/cma.j.issn.1002-0098.2018.01.009
- VernacularTitle: 载血管内皮细胞生长因子微球促进牙髓再生和血管形成的实验研究
- Author:
Xiangwei LI
1
;
Hongchen SUN
2
;
Xiaohua LIU
3
Author Information
1. Department of Endodontics, School of Stomatology, Jilin University, Changchun 130021, China
2. Department of Pathology, School of Stomatology, Jilin University, Changchun 130021, China
3. Department of Biomedical Sciences, Baylor College of Dentistry, Texas A&M University, Dallas TX 75246, U S A
- Publication Type:Journal Article
- Keywords:
Dental pulp;
Vascular endothelial growth factor;
Pulp regeneration;
Dental pulp stem cells
- From:
Chinese Journal of Stomatology
2018;53(1):42-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of vascular endothelial growth factor (VEGF)-loaded microspheres on dental pulp tissue regeneration and vascularization in vivo.
Methods:In vitro release experiment and human umbilical vein endothelial cell migration experiment were conducted with VEGF loaded microspheres. The dental pulp stem cells (DPSC) were co-cultured with VEGF microspheres to observe the compatibility between the cells and the microspheres. DPSC and VEGF loaded microspheres were injected into the root lumen through the apical foramen, which were then transplanted subcutaneously into nude mice. Histological and immunohistochemical features were observed after nine weeks.
Results:DPSCs attached and spread on the surface of the microspheres. HE staining showed that the regenerated pulp-like tissue fulfilled the whole apex and middle third of the root. Differentiated odontoblast-like cells aligned with the existing tubular root dentin.
Conclusions:VEGF-loaded microspheres promoted the regeneration of pulp-like tissues and formation of blood vessels.
