Experimental study of miRNA200a regulating Wnt/β-catenin signaling pathway in silica-induced mouse lung epithelial cells
10.3760/cma.j.issn.1001-9391.2018.01.014
- VernacularTitle: miRNA200a调控二氧化硅诱导小鼠肺上皮细胞Wnt/β-catenin信号通路的相关基因表达
- Author:
Xin WANG
1
;
Qiang ZENG
;
Xueying YANG
;
Jing LIU
;
Yitao LIU
Author Information
1. Tianjin Centers for Disease Control and Prevention. Tianjin 300011, China
- Publication Type:Journal Article
- Keywords:
miRNA200a;
silica;
pulmonary epithelial cells;
Wnt/β
-catenin signaling pathway
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2018;36(1):47-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of overexpression of miRNA200a (miR-200a) recombinant lentivirus on the expression of Wnt/β-catenin signaling pathway in mouse lung epithelial cell line MLE-12 induced by silica (SiO2) .
Methods:The mice were divided into SiO2 control group (SiO2) , virus control group (SiO2+Lv-NC) group and overexpressing miR-200a virus group (SiO2+Lv-miR-200a). The expression of β-catenin, MMP2, MMP9, TCF-4 and Cyclin D1 mRNA and protein were detected by realtime-PCR and western blot after incubating cells for 18 h stimulating at the final concentration of 200 μg/ml of SiO2.
Results:The expression of miR-200a in MLE-12 cells of SiO2+Lv-miR-200 a group was significantly higher than that in SiO2 group and SiO2+Lv-NC group. The mRNA and protein expression of β-catenin, MMP2, MMP9, TCF-4 and Cyclin D1 in MLE-12 cells of SiO2+Lv-miR-200a group were significantly lower than those in SiO2 group and SiO2+Lv-NC group (P<0.05) .
Conclusion:Overexpression of miR-200a can inhibit the expression of related genes of Wnt/β-catenin signaling pathway in silica-induced mouse lung epithelial cells.
