Advances in strategies for activating silent biosynthetic gene clusters in Streptomyces
10.11665/j.issn.1000-5048.20190401
- VernacularTitle:链霉菌沉默生物合成基因簇激活策略的研究进展
- Author:
Yan DAI
1
;
Xuri WU
;
Yijun CHEN
Author Information
1. 中国药科大学生命科学与技术学院化学生物学研究室
- Publication Type:Journal Article
- Keywords:
Streptomyces;
secondary metabolites;
silent biosynthetic gene clusters;
bioinformatic tools;
gene clusters activation strategies
- From:
Journal of China Pharmaceutical University
2019;50(4):379-388
- CountryChina
- Language:Chinese
-
Abstract:
Microbial secondary metabolites have always been one of the important sources of discovery and development of new drugs due to their remarkable biological activities. The explosion of genome sequences has revealed that Streptomyces harbor an immensely untapped biosynthetic potential. However, the number of active secondary metabolites with new skeletons or structural units found from Streptomyces is much lower than that of biosynthetic gene clusters(BGCs), mainly due to the fact that many BGCs are either expressed weakly or transcriptionally silent under conventional laboratory conditions. Beginning with the bioinformatics tools for BGCs prediction, this review focuses on the classical approaches to activate silent BGCs of Streptomyces in native and heterologous hosts. Moreover, several new strategies including transcriptional factors decoy, reporter-guided high-throughput selection and muliplexed CRISPR-TAR were detailed, which provide methodological references for mining new secondary metabolites from Streptomyces.
