MALAT1 upregulates SMYD3 by competition with miR-124 and promotes proliferation and migration of breast cancer cells
10.11665/j.issn.1000-5048.20190311
- VernacularTitle:MALAT1通过竞争miR-124上调SMYD3并促进乳腺癌细胞增殖与迁移
- Author:
Manli XU
1
;
Chang WANG
;
Nan WANG
;
Hongpeng HE
;
Tongcun ZHANG
;
Xuegang LUO
Author Information
1. 天津科技大学生物工程学院，工业发酵微生物学教育部重点实验室，天津市工业微生物重点实验室
- Publication Type:Journal Article
- Keywords:
breast cancer cells;
lncRNA MALAT1;
SMYD3;
miR-124
- From:
Journal of China Pharmaceutical University
2019;50(3):344-351
- CountryChina
- Language:Chinese
-
Abstract:
To investigate whether lncRNA MALAT1 affects the migration and proliferation of breast cancer cells through the regulation with histone methyltransferase SMYD3, the endogenous MALAT1 in the MCF-7 and MDA-MB-231 breast cancer cells were knocked down by siRNA, and then the migration and proliferation of cells were detected by wound healing migration and MTT assay. The effects of si-MALAT1 on the mRNA and protein levels of miRNA-124, SMYD3 and its downstream genes were detected by Real time PCR and Western blot. The results showed that siRNA-targeted knockdown of MALAT1 reduced the migration and proliferation of breast cancer cells, and inhibited the transcriptional expression of SMYD3 and its downstream genes, including N-cadherin, MYL9, MMP9 and CYR61, and up-regulated miR-124. Overexpression of miR-124 reduced the expression of SMYD3 in breast cancer cells, and knockdown of MALAT1 attenuated the promotion of SMYD3 protein expression by miR-124 inhibitors. In addition, SMYD3 overexpression activated MALAT1 transcription, whereas siRNA interference with SMYD3 downregulated MALAT1. These results indicate that LncRNA MALAT1 acted as a competing endogenous RNA(ceRNA)of miR-124 to regulate expression of SMYD3 in breast cancer cells, and SMYD3 can activate the transcription of MALAT1, which will affect the proliferation and migration of breast cancer cells.
